REQUIREMENT OF CAPACITATION FOR SPERM PENETRATION OF ZONA-FREE RAT EGGS
نویسندگان
چکیده
منابع مشابه
Ethylene glycol-supplemented calcium-free media improve zona penetration of vitrified rat oocytes by sperm cells.
Cryopreservation of matured oocytes is a useful technique because the oocytes can be used for some assisted reproductive technologies after warming. Even though rats, like mice, have been used in various research fields including reproductive technology, information about cryopreservation of rat oocytes is limited. The objective of the present study was to improve the vitrification protocol for...
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A previous study (Takahashi & Hanada, 1984) reported the penetration of zona-free hamster eggs by ejaculated bull spermatozoa after treatment with calcium ionophore A23187 (Ca-IA) in the presence of caffeine. Based on this study, successful IVF was accomplished not only in cattle (Hanada, 1986b) but also in goats and sheep (Hanada, 1986a) using spermatozoa treated with Ca-IA. In the present stu...
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In most mammals that have been studied, the first spermatozoon which penetrates an egg stimulates the egg to undergo a reaction—the 'zona reaction'—which bars further spermatozoa from penetrating through the zona pellucida (Braden, Austin & David, 1954). The sequel to the zona reaction may be classified into two categories, according to species: species in which spermatozoa are entirely prevent...
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ژورنال
عنوان ژورنال: Reproduction
سال: 1975
ISSN: 1470-1626,1741-7899
DOI: 10.1530/jrf.0.0440305